During cellular stress, global translation is inhibited, and mRNA is phase-separated into cytosolic membrane-less organelles. Stress granules (SG) form during stress, while P-bodies (PB) are endogenously present and expand during stress. This response allows for prioritised translation of stress-specific genes, as excess mRNAs are translationally repressed inside the granules. As a result, cancer cells harness this mechanism to limit energy expenditure and survive chemotherapy. However, it is currently unclear how SG and PB individually contribute to translational repression during stress. Previous studies have observed interaction and mRNA exchange between the organelles, whilst others have struggled to differentiate their functions. This study combines high-content imaging with sub-cellular RNA localization analysis to inspect the individual dynamics and composition of SG and PB during stress. This approach revealed unique morphological dynamics for SG and PB from early to late stress, suggesting each organelle operates differently. In line with this, the RNA composition of SG and PB differed during late stress, indicating potentially distinct roles in RNA regulation. Live imaging was also used to identify significantly increased SG-PB interaction during late stages of the stress response. These results outline clear differences between SG and PB during the stress response, both in morphology and RNA composition, implying distinct roles in post-transcriptional regulation and cellular adaptability.